Part:BBa_K152006:Design

frdBCD operon from E. coli (missing the first gene fumarate oxidase)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1207
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

FrdA was not included in the production of this biobrick, on account of it containing several Biobrick sites; too many to remove through site directed mutagenesis. Hopefully FrdBCD is enough to induce the membrane doubling phenotype.

Source

E. coli DH5 alpha genomic DNA.